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Interestingly, the mesenchymal shift by Day 45 suggests greater cellular complexity at later stages, potentially facilitating iHLO use in modeling liver pathologies such as fibrosis, cirrhosis, and liver remodeling, as indicated by increased expression of hepatic stellate cell markers such as DES and VIM 33,34

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In TRPM7-CKO mice, TRPM7 expression was significantly reduced in the hippocampus, compared to floxed controls, as confirmed by western blot (Fig

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