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Although it remains unknown as to how the inhibition of the interaction with BRD4 occurs in the context of full-length ASXL1, the intramolecular contacts with the C-terminal region of ASXL1 that occlude ASXL1 EBM could play a role

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After the supernatants were incubated with JC-1 (0.5 mol/L, Invitrogen) at room temperature, the samples were precipitated by centrifugation at 600 g and 4 C for 3 min

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