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The aggressiveness of Cu + destroys the lysosomal membrane with release of Cu + to cytoplasm and consequent hepatocellular injury, furthermore the plasma membrane becomes leaky with loss of Cu + to blood and eventually destruction of erythrocyte membranes (hemolysis)

The protein sample (5-30ug) is diluted with 2X sample loading buffer and denatured by heating for 5 min between 70 and 100

The procedure doesnt take more than 45 60 minutes to complete

Niacinamide is responsible for fulfilling vitamin B3 requirements that lead to neurological problems like memory loss, headache, etc

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