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Cystine restriction rescues glutamine-addicted TNBC cells from glutamine deprivation by restoring intracellular TCA cycle intermediates Given that TNBC cells exhibit heightened reliance on extracellular glutamine due to enhanced glutamine anaplerosis, we next investigated whether this phenotype is linked to an increased dependence on extracellular cystine

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Moreover, peroxynitrite has been implicated in the nitration of the EAAC1 neuronal transporters, reducing the cells ability to take up cysteine and inhibiting the intracellular neuronal production of the endogenous antioxidant glutathione, which can also lead to cell death due to the inability to counter intracellular oxidative stress (McElroy et al., 2017)