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Next day, samples were washed with Ca 2+ /Mg 2+ PBS and incubated for 2 h at room temperature with secondary antibodies, conjugated with AlexaFluor 488 (Ex/Em = 495/519 nm, dilution in PBS to the concentration 4 g/mL, Abcam Limited, Discovery Drive, Cambridge Biomedical Campus, Cambridge, United Kingdom, ab150077)

Landau & Tucker (1984) found the 48-h LC 50 values for sheepshead minnow Cyprinodon variegautus and snook Centropomus undecimatis, both estuarine fish, to be 4.8 and 6.2 mg/litre, respectively

"Efficacy and safety of cagrilintide for weight management: A randomized phase 2 trial." Obesity , 31(3), 687-699

Excess cobalamin in the blood showed barely detectable binding to TC II (Andrs et al., 2013)