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Louis, MO) and 5% milk (LabScientific Inc., Highlands, NJ) and incubated overnight with the following primary antibodies in TBST (Tris-buffered saline, 0.1% Tween-20) containing 5% BSA and 0.03% sodium azide: Cell SignalingpAkt (S473) (Cat#: 4058), pERK1/2 (T202/Y204)) (Cat#: 4370), glyceraldehyde 3-phosphate dehydrogenase (Cat#: 91766)

Additionally, immunofluorescence experiments indicated that under senescent conditions, p-P53 primarily accumulated in the cell nucleus (Figure 5E)

USA 89 , 1157411578 (1992)