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Abbreviations ATF4: Activating transcription factor 4 m: Mitochondrial membrane potential DCFH-DA: 2,7-dichlorofluorescin diacetate ECL: Enhanced chemiluminescence ELISA: Enzyme-linked immunosorbent assay ESI: Electrospray ionization FC: Fold change Fe: Ferrous iron Fer-1: Ferrostatin-1 GBM: Glioblastoma multiforme GSH: Glutathione HMDB: Human metabolome database HRP: Horseradish peroxidase IC50: Half-maximal inhibitory concentration KEGG: Kyoto Encyclopedia of Genes and Genomes K: Potassium ion LC-MS/MS: Liquid chromatographytandem mass spectrometry LDH: Lactate dehydrogenase Lip-1: Liproxstatin-1 MDA: Malondialdehyde Nrf2: Nuclear factor erythroid 2related factor 2 OH: Hydroxyl radicals PBS: Phosphate-buffered saline PCA: Principal component analysis PLS-DA: Partial least-squares discriminant analysis PVDF: Polyvinylidene difluoride QTOF: Quadrupole time-of-flight mass spectrometer Rb: Rubidium ion RbCl: Rubidium chloride RCD: Regulated cell death RIPA: Radioimmunoprecipitation assay ROS: Reactive oxygen species RT: Retention time SDS-PAGE: Sodium dodecyl sulfatepolyacrylamide gel electrophoresis TCA: Tricarboxylic acid TEM: Transmission electron microscopy TMZ: Temozolomide UPLC: Ultra-performance liquid chromatography References Louis DN, Perry A, Wesseling P, Brat DJ, Cree IA, Figarella-Branger D, et al

10.1038/nature05859 187 YanB.AiY.SunQ.MaY.CaoY.WangJ.et al (2021a)

Whole-blood antioxidant capacity and the RBC GSH/GSSG ratio were chronically decreased after TBI