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Subsequently, all samples were subjected to centrifugation at 1500 rpm for 15 min at 4 C, facilitating a clear delineation of the two phases: the upper (lipophilic) phase, which contains the apolar metabolites, and the lower (hydrophilic) phase, which comprises the polar metabolites of interest

Kandemir FM, Caglayan C, Aksu EH et al (2020) Protective effect of rutin on mercuric chloride-induced reproductive damage in male rats

Thus our assay allows us to separate and identify M