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The subsequent design of compounds aimed at targeting these newly identified sites will facilitate the discovery of novel antiviral drugs

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( G ) Lung metastatic cell lines (PDAC) stably expressing ipCW, ipCW- Gstt1 wild-type and ipCW- Gstt1 R234G were treated with doxycycline for 5 days

Briefly, the supernatant is loaded onto the column in 6 M guanidine-HCl, pH 8, the column is first washed with 10 volumes of 6 M guanidine-HCl, pH 8, then washed with 10 volumes of 6 M guanidine-HCl pH 6, and finally the polypeptide is eluted with 6 M guanidine-HCl,pH5