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Conditioned medium experiment

Gottfried C et al (1999) Regulation of protein phosphorylation in astrocyte cultures by external calcium ions: specific effects on the phosphorylation of glial fibrillary acidic protein (GFAP), vimentin and heat shock protein 27 (HSP27)

After 2 weeks, animals with blood glucose values above 180 mg/dL were selected to perform the tests described below