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For this purpose, mitochondria were placed in the incubation medium of isotonic composition (mmol/L): KCl120, Tris-HCl25, KH 2 PO 4 3, sodium succinate5, pH 7.4, and a decrease in the optical density in mitochondria suspension was recorded at = 520 nm for 15 min of the mitochondria swelling

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[DOI] [PubMed] [Google Scholar] 278.Babizhayev M.A., Deyev A.I., Yermakova V.N., Semiletov Y.A., Davydova N.G., Doroshenko V.S., Zhukotskii A.V., Goldman I.M

(2025) As mentioned earlier, though protein binding was recognized early as the initial step in APAP hepatotoxicity, mechanisms of subsequent free radical formation were controversial