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Phosphate buffered saline (without Ca2+ and Mg2+) containing 5% fetal calf serum (FCS) 5 mM EDTA and 5 g/ml DNase I

Impairment of lipophagy by PNPLA1 mutations causes lipid droplet accumulation in primary fibroblasts of Autosomal Recessive Congenital Ichthyosis patients

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For testing efficacy of peptide inhibitor, A 40 was premixed with equal volume of PBS or peptide as indicated, and incubated for 24 h at room temperature with shaking (50 rpm) before being added to the cultures