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Meanwhile, in the HACE model induced by low-dose lipopolysaccharide (LPS, 0.5 mg/kg) combined with acute hypobaric hypoxia exposure (6000 m, exposure for 24 h), the level of TNF-, IL-1, and IL-6 in plasma and hippocampal tissue increased significantly, and the brain water content increased

The AlamarBlue (ThermoFisher Scientific, Waltham, MA, product number DAL1025) staining was performed according to the manufacturers instructions: 10 l of the reagent was added per well and the reading of 560/590 nm fluorescence commenced, using ThermoFisher Fluoroscan plate reader

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First, a band is tied around your upper arm so the vein fills with blood

Yao Q, Zhai Y, He Z, Wang Q, Sun L, Sun T, et al