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Epithelial-mesenchymal transition during extravillous trophoblast differentiation

Individual fish were divided using a razor blade into 0.5 cm sections and added to a 40 mL glass tube along with 0.1 mL of DI water (18 M-cm Milli-Q, EMD Millipore, Burlington, MA) whereupon tissues were homogenized into a paste using an Omni TH (Omni Int., Kennesaw, GA) tissue homogenizer

Their activation leads to varying amplitude and dynamic changes in neuronal excitability, promoting ATP release and preventing neuronal overexcitement

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