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Dithiothreitol (60 mmol/L in 0.1 mol sodium tetraborate/L) was added to convert oxidized glutathione (GSSG) to GSH and was then alkylated by adding iodoacetamide (0.5 mol/L) in 0.1 mol ammonium bicarbonate/L

By contrast, other evidence indicates that Cu+ may promote the formation of soluble toxic oligomers or redox cycling with Cu2+, contributing to oxidative stress and aggregation (192,193)

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