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(D) ER stress-related proteins were measured by western blot under the treatment of MHO7 (+: 2 M

Antigen characterization p/p*MHCs stability analysis Stability of p*MHCs in vitro was analyzed by multiplex bead binding assay 61 , as follows: Biotinylated p*MHCs were immobilized at 5% nominal saturation to beads (Dynabeads M-270 Streptavidin, Thermo Fisher, 65305) labeled with different concentrations of DyLight 650 (Thermo Fisher, 84547) to allow pooling of multiple samples

2) that could be targeted by antibodies, specifically at the apex of the structure where the loop region of C of DIII is located and at the fusion loops near the membrane

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