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Redundancy in bacterial resistance to ROS is a relatively common phenomenon, including the need to eliminate five individual enzymes in Salmonella enterica Serovar Typhimurium to exhibit a phenotype in the presence of hydrogen peroxide (Hbrard et al., 2009) and simultaneous disruption of four enzymes in Bacillus anthracis to observe a phenotype in the presence of superoxide (Cybulski et al., 2009)

Then, 500 L of the supernatant was removed, 500 L of isopropyl alcohol was added (supernatant:isopropyl alcohol = 1:1), the mixture was allowed to stand for 5 min, and the mixture was centrifuged at 12,000 rpm at 4 for 10 min

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