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Due to this heterogeneity, the reported tetrameric state of CBS is likely to be two dimers interacting through a single Bateman module pair but additionally could also be formed via the degradation of the Bateman module that we have shown here (Supplementary Fig

After three washes with PBS (5 min each), biotin-labeled goat anti-rabbit IgG was added and incubated at 37C for 50 min

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