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Patel et al., 2012)

Review of the pharmacological effects of astragaloside IV and its autophagic mechanism in association with inflammation

Circular Dichroism (CD) Spectroscopy Analysis of Secondary Structure of refolded Pure Protein Transfer 0.5 - 1 mg of refolded protein obtained in step 6.6 into a dialysis tube and place it in a dialysis bucket containing 5 L of buffer L (50 mM sodium phosphate pH 7.4), precooled to 4 C