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Cells were starved for nitrogen in a carbon free M9 medium for 3 h at 37 C, harvested by centrifugation and adjusted to the optical density of 3.0 in 20 mM TBS buffer (pH-7.2)

Consistently, other studies also confirmed that autophagy inhibition increases ROS production in Pca cells (7577)

Ayton S, Wang Y, Diouf I, Schneider JA, Brockman J, Morris MC et al (2020) Brain iron is associated with accelerated cognitive decline in people with Alzheimer pathology

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Abstract Inflammatory bowel disease (IBD) is a chronic, relapsing gastrointestinal (GI) disorder characterized by intestinal inflammation