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Himar-1 transposon transduction was selected on erythromycin (Sigma-Aldrich) after two days

L.EsiriM

In the final step to the coenzyme, CobC removes the 5' phosphate group: [32] [33] Adenosylcobalamin-5'-phosphate + H 2 O adenosylcobalamin + phosphate The complete biosynthetic route involves a long linear path that requires about 25 contributing enzyme steps