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Cells were washed and incubated with Cy-3 or Cy-5 conjugated secondary antibodies (Jackson ImmunoResearch Laboratories, West Grove, PA and Thermofisher)

We therefore suggest that our findings are valid representations of the relativities of each parameter among strains, temperatures and time-points, and their various interactions, whilst also recognizing the additional value in calibrating our methodology against absolute quantifications of the underlying biochemistries in future studies

The cells were then washed with PBS buffer, and 1 10^6 cells were taken for each sample

Dopamine, 6-hydroxydopamine, iron, and dioxygentheir mutual interactions and possible implication in the development of Parkinsons disease

GlycoATP and mitoATP were derived from cells sequentially treated with oligomycin A and Rot/Antimycin A