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Description

Fruitful developments have also occurred by accident

Apoptosis and acute kidney injury

Reverse transcription reactions (20 l) were carried out with SuperScript III reverse transcriptase (Thermo Fisher, 18080085) according to the manufacturers instructions and containing 4 l 5x first-strand buffer, 1 l of murine RNase inhibitor (NEB, 174M0314S), 1 l of 10 mM dNTP mix (Thermo Fisher, 18427-013), 50 ng of random hexamers (Bioline, BIO-38028), and 1 g of total RNA (in a total volume of 11 l)