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Gels were homogenized with a polypropylene EPPI-pestle homogenizer in the eppendorf tube for 10 to 20s at room temperature until most of the homogenate pieces were small enough to pass through a 1 mL micropipette tip

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Unlike synthetic drugs, the topical application of polyherbal formulations for wound treatment has generally not shown any side effects, such as skin irritation, toxicity, erythema, eschar, or edema during acute dermal toxicity and skin irritation tests in animal burn wound models

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The frequency: For maintenance (for example, preventing LPP flares), dosing may be as infrequent as once every few months