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They involve a quick needle prick followed by a relaxed sitting period

Under the influence of biological activity, chemical reactions, and physical forcesincluding hydrolysis and ultraviolet exposureplastic debris gradually fragments, giving rise to MPs/NPs 2,3,4

The plates were incubated for 30 min at 25C before the addition of 100 l of the substrate solution (0.04 M Na 2 HPO 4 , 0.2 mm, 5,5-dithio-bis-2-nitrobenzoic acid (DTNB), 0.24 mm acethyltiocholine iodide (ATCI) in HPLC-grade water

Storage keep sealed in a cool dry dark place - product from lights and moisture

Based on these limitations, future research should integrate detailed exposure metrics, genetic profiling, and chronotype assessments to better clarify mechanisms and identify high-risk subgroups