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(April 2005)

1B), and then maintained for 5 days in OL differentiation medium (DM) containing DMEM (Thermo Fisher), 1% P/S (Thermo Fisher), 1 mM sodium pyruvate (Sigma), 0.1% bovine serum albumin (Sigma), 50 g/ml apotransferrin (Sigma-Aldrich), 5 g/ml insulin (Sigma-Aldrich), 30 nM sodium selenite (Sigma), 10 nM biotin (Sigma-Aldrich), 10 nM hydrocortisone (Sigma-Aldrich a), 15 nM triiodothyronine (Sigma), 10 ng/ml ciliary neurotrophic factor (Cell Guidance Systems), and 5 g/ml N-acetyl-cysteine (Sigma-Aldrich)

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