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mRNA analysis by RT-PCR Frozen intestinal mucosal samples (~ 100 mg) were powdered and homogenized, and total RNA was isolated by using the TRIzol Reagent protocol (Invitrogen Technology, Carlsbad, CA, USA)

Even so, a clear mechanism in the lab is not the same as proven results in people, and we will not promise an outcome the science has not established

Short-chain fatty acids (SCFA) Short-chain fatty acids (SCFAs) are products of bacterial metabolism, deriving from the fermentation of indigestible fibers

Future studies should determine whether hormonal factors mediate this sex-specific sensitivity (Buchenauer et al., 2023)

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