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Briefly, for chromatographic separation of amino acids and acylcarnitines, a 2.1 100 mm, 100 , 1.7 m, UPLC BEH amide column (Waters, Eschborn, Germany) was used with 5 mM ammonium acetate in water (eluent A) and 5 mM ammonium acetate in acetonitrile/water (95/5, v/v) (eluent B) as elution solvents both adjusted to pH 3 using acetic acid

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Abbreviations ALT: Alanine transaminase AST: Aspartate transaminase RPM: Revolutions per minute H&E: Hematoxylin-eosin LC-MS/MS: Liquid chromatography coupled to tandem mass spectrometry (LC-MS/MS) QQQ: Triple quadrupole QTRAP: A triple quadrupole-linear ion trap mass spectrometer KEGG: Kyoto encyclopedia of genes and genomes MSEA: Metabolite sets enrichment analysis MPO: Myeloperoxidase PCA: Principal component HCA: Hierarchical cluster analysis PCC: Pearsons correlation coefficients POD: Postoperative day References Michalopoulos GK, Bhushan B

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