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Da 7 , Renee-Claude Mercier 7 , Audrey H

Numerous studies have investigated the kinetic behavior of linear and macrocyclic complexes [18,19,20]

Unlike conventional Cas9, dCas9 lacks endonuclease activity due to mutations in the RuvC1 (D10A) and HNH (H840A) domains, making it ideal for gene repression (CRISPRi) or activation (CRISPRa) when fused to transcriptional effectors such as KRAB, SRDX, or VP64 [150]