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U-100 calibration (100 units per ml) Appropriate needle gauge and length per Section 7 below 70% isopropyl alcohol swabs at least two per injection (one for vial top, one for skin) Sterile gauze or cotton ball for post-injection pressure FDA-compliant sharps disposal container a rigid, puncture-resistant container with a locking lid

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Phospho-Ku70 induced by DNA damage interacts with RNA Pol II and promotes the formation of phospho-53BP1 foci to ensure optimal cNHEJ

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