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The occurrence of a primary injury is often followed by a series of secondary injuries, including disruption of the bloodspinal cord barrier, neuroinflammation, and oxidative stress (OS)-induced damage, among others, and this phase may last for extended periods [1, 2]

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Lipids 40 , 987994 (2005)

Membranes were washed twice with water and incubated overnight at 4 C in Tris-buffered saline with 0.1% Tween-20 (TBSt) containing 3% BSA (Sigma-Aldrich) and primary antibodies