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The medium was removed following treatment, and the cells were incubated for 30 min at 37C in a buffer containing 10 M of H2-DCFDA

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"Patients with IDH1 wild type anaplastic astrocytomas exhibit worse prognosis than IDH1-mutated glioblastomas, and IDH1 mutation status accounts for the unfavorable prognostic effect of higher age: implications for classification of gliomas" (PDF)