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The erythrocytes were then lysed with lysis buffer (BD Pharm Lyse, BD Bioscience, San Jose, USA) for 10 min followed by centrifugation (250 x g, 5 min, 4C, Centrifuge 5427 R, Eppendorf SE, Hamburg, Germany) and resuspension of the cells in Hepes-buffered saline (HBS, 14 mM Hepes, 0.9% NaCl)

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In contrast, a negligible difference in GFP - ACA11-F3 half-life was observed when it was co-expressed with either TZF1 or TZF1 H186Y , in agreement with our observation of specific binding of TZF1 to the F1/F4 region of ACA11 in RIP-qPCR experiments ( Figures 6D, E )

[40] [41] Their next album would be recorded with producer John Feldman