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Likewise, while many evolutionary changes may be related to the generation of new genes, it is broadly recognized that the bulk of interspecies differences arise corollary to changes in gene activity

This concern may have originated from a 1959 study that first raised the possibility of diuretics affecting glucose metabolism (Freis and Finnerty, 1959) based on the effects of hydrochlorothiazide

Zalevsky, J

Cell 158 , 481491 (2014)

Solid YPD medium containing 200 g ml -1 hygromycin was used for selection of gsh2 deletion mutants, and medium containing 100 g ml -1 nourseothricin was used to select for the gsh2::GSH2 reconstituted strain in the gsh2 mutant background