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Samples were kept chilled during transport, were centrifuged (3000 rpm for 15 min) and plasma aliquots were labelled and stored at 80C until required for analysis

Tirzepatide also suppresses glucagon secretion, slows gastric emptying, and reduces appetite through central nervous system pathways, contributing to both glycaemic control and weight reduction

This will subsequently form complexes with the cobalmin and thereby reduce its efficacy