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Using spatial transcriptomics, GPX4 has been identified to be located at the interface between the inner cortex and the outer medulla of the kidney, which is the main site of cell ferroptosis after I/R injury

Methods: Healthy human PCLS were cultured for up to 96 hours in either GFIPO medium (Williams E Medium supplemented with Glucose (36 mM), Fructose (5 mM), Insulin (1 nM), Palmitic acid (0.24 mM) and Oleic acid (0.48 mM)) to mimic NAFLD-inducing conditions, or WEGG medium (Williams E Medium supplemented with Glucose (25 mM)) as a control

Vitaly Pozdeev (Uni.Lu), Dominique Revets (LIH), Thomas Cerutti (LIH), and Gert Van Isterdael (VIB) for tissue section microscopy and FACS sorting

Ahmadi Asouri S, Asemi R, Aghadavod E, Jamilian M