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Studies in mice revealed that dietary supplementation with TMAO, choline, or carnitine decreased reverse cholesterol transport (RCT) (Janeiro et al., 2018), a mechanism that counteracts excess cholesterol deposition in peripheral tissues by transporting excess cholesterol to the liver and small intestine (Ross and Glomset, 1973).The major pathway for cholesterol elimination is the metabolic synthesis of bile acids in the liver (Zarate et al., 2016), and it was reported that the size of the total bile acid pool was significantly smaller in mice administered TMAO (Koeth et al., 2013)

Previous clinical investigations indicated that ALOX5 expression was positively correlated with the risk of MI and the inflammatory state of white adipose tissues in obese and diabetic women

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eLife, 14, e12270663

Samples were tested in triplicates in three separate experiments