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Measurement of oxidized glutathione and reduced glutathione To measure GSH, 10L of saliva were incubated with 10L of a solution of ethanol o-phthaldehyde (OPT, 1mg/mL) and 180L of phosphate buffer (sodium phosphate 100mM, 2.5mM EDTA-Na2, pH 8.0) for 15min at room temperature

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DSB detection was based on expression levels of -H2AX, a classic biomarker for DSBs, which could be visualized by immunofluorescence microscopy

Immune and Hormonal Modulation: Functional foods and exercise enhance immune surveillance and reduce circulating estrogens, critical in hormone-sensitive BC subtypes

Allele frequencies for genetic polymorphism are such that ~1 in 300 Caucasians is homozygous for a defective allele or alleles for the trait of very low activity, ~11% of people are heterozygous and have intermediate activity