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gBlocks containing a FLAG tag followed by the full coding sequence of human CDKN1A , the N terminus only (amino acids 190), or the C terminus (amino acids 87164) were synthesized by IDT DNA and cloned into lentiviral vectors containing a Tet-inducible promoter (pLenti-CMV-TRE3G-Puro, a gift of Jan Carette) by Gibson assembly

Feinberg, A

That means some women will have symptoms related to high estrogen levels, like heavy, painful periods and symptoms of PMS, until just before menopause

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