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Progressive serum HBsAg reduction was closely paralleled with progressive serum HBeAg decline, implying that progressive serum HBsAg reduction reflects progressive HBV clearance in the livers, which was confirmed by a 3-4 log lower intrahepatic HBsAg, rc, and cccDNA levels from mice who achieved undetectable serum HBsAg compared to untreated controls

A CoA for a four-component blend faces a higher verification burden: it must document purity and identity for each component separately, confirm the blending ratio, and ideally provide both HPLC chromatogram data and LC-MS identification data for each peak