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This makes SubQ administration suitable for studies aiming for a rapid, albeit very brief, systemic pulse of GHK-Cu

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Materials and methods Cell culture The human trophoblast cell line HTR8 was kindly provided by East China Normal University (ECNU) and cultured in RPMI 1640 medium supplemented with 10% fetal bovine serum (FBS) and 1% penicillin-streptomycin

Cells were cultured using AIM-V (Thermo Fisher Scientific) supplemented with 10% FBS, 100 U ml 1 penicillin, 100 g ml 1 streptomycin and 2 mM l-glutamine under humidified conditions in 5% CO 2 at 37 C