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Approximately 50 mg of frozen cecal content was added to 0.1 mm glass bead-containing tubes (PowerBead Tubes, Qiagen) prefilled with 300 l RLT buffer (RNeasy mini kit, Qiagen) supplemented with -mercaptoethanol (10 l/ml, Sigma-Aldrich) and 1 ml TRIzol (Invitrogen)

844 Ferroptosis in intestinal ischemia/reperfusion injury Intestinal ischemia/reperfusion (I/R) injury occurs in many clinical conditions, such as acute mesenteric ischemia, small intestine torsion, and trauma, and is a life-threatening vascular emergency

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doi: 10.1016/j.biotechadv.2013.06.007, 78 NapoleoniM.KlennerF.KhawajaN.HillierJ