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Mitochondrial glutamine metabolism is a significant anaplerotic step in tumorigenesis

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CRISPR-Cas9 knockout screen confirms the role of LCS3 in mediating antioxidant response and reveals suppression of NQO1 as a mechanism of resistance To complement the effort to identify targets of LCS3 with TPP and to interrogate potential mediators of the LCS3 response systematically, we performed a genome-wide, loss-of-function CRISPR-Cas9 screen in LCS3-sensitive LUAD cells