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A novel paradigm for heart failure with preserved ejection fraction

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GST-OLFM4 protein was expressed in BL21 (DE3) by 0.5 M IPTG inducing overnight, then harvest cells and resuspend in phosphate-buffered saline (PBS) (0.1% Triton100, Protease Inhibitor Cocktail, 1 mM PMSF), after lyzed by sonication, proteins were purified by glutathione Sepharose 4B (GE healthcare)