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NAD(P)H autofluorescence was measured with excitation at 340 7.5 nm and the emission was detected at 445 10 nm

Given the extended literature reporting the influence of pubertal hormones on HPA-axis [49], redox regulation [50, 51] and brain maturation [11], we explored in a secondary hypothesis whether the aforementioned associations were related to pubertal status and gonadal hormones (estradiol and testosterone)

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For fluorescence staining of tissues, the slides were incubated overnight at 4 with antibodies against CD80 (Santa Cruz Biotechnology, Santacruz, CA, USA) and SLC25A33 (Origene, MD, Rockville, USA)

Make sure the black dot is facing towards you, and that you snap the lid away from you