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PCR reaction was set using 50 ng cDNA, 10 l SyBr Green Premix Ex Taq II (Tli RNaseH Plus) (Clonetech, Japan) and 10 pmol each of forward and reverse primers in 20 l reaction volume

Conversely, higher 25-hydroxy vitamin D levels had a protective role against asthma symptoms in homes with increased PM 2.5 , with estimated OR of 0.87