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pH 7.2 adjusted with HCl and NaOH with 0.1 dilution of trace element solution (41 mM CaCl 2 , 22 mM FeSO 4 , 6 mM MnCl 2 , 3 mM CoCl 2 , 1 mM ZnSO 4 , 0.1 mM CuCl 2 , 0.2 mM (NH 4 ) 6 Mo 7 O 24 , 17 mM EDTA) with kanamycin (25 mg l-1) in which 15 N-NH 4 Cl (2 g l-1) was the only source of nitrogen for NMR isotope labelling purposes, respectively (Cambridge Isotope Laboratories, Inc)