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However, inducing angiogenesis should be undertaken with caution, as inducing the formation of new blood vessels in areas where cancer cells are still present can facilitate tumor growth and could allow the cells to enter the systemic blood flow, with the possibility of inducing metastasis (Muz et al., 2015)

While this was a useful observation and allowed many lines to be assessed by RT-qPCR, it still required validation by further in vitro differentiation