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This increase implies that these miRNAs have significant regulatory functions throughout the early stages of embryonic development and during the activation of the genome [219, 221], Early bovine blastocysts showed increased expression of miR-135a, miR-218, miR-335, and miR-449b in comparison to hatched ones [264], The mouse embryos dynamic and strictly controlled expression patterns of miRNAs throughout preimplantation development are best shown by the downregulation of miR-182 between the 2- and 4-cell stages and the subsequent increase between the 4- and 8-cell stages

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