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Effect of ascorbic acid-Fu-30 on Pseudomonas aeruginosa adhesion and biofilm formation Pseudomonas aeruginosa isolates were inoculated to an optical density (OD 600 nm ) = 0.1 0.02 and allowed to grown in 20% MHB in the presence or absence of ascorbic acid (20 mM) + Fu-30 (20 M) for 2, 8 and 24 h at 37C with shaking (100 rpm) in 6-well plates

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Western blot analysis confirmed a significant reduction of Ssu72 protein expression in eWAT, inguinal white adipose tissue (iWAT), and brown adipose tissue (BAT) of Ssu72 aKO mice (Supplementary Fig